Browsing Faculty of Health Sciences (FHS) by Author "Hand, G"
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The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of bacillus circulans xylanase.
McIntosh, LP; Hand, G; Johnson, PE; Joshi, MD; Körner, M; Plesniak, LA; Ziser, L; Wakarchuk, WW; Withers, SG (University of NairobiFaculty of medicine, 1996-08)The 20 kDa xylanase from Bacillus circulans carries out hydrolysis of xylan via a two-step mechanism involving a covalent glycosyl-enzyme intermediate. In this double-displacement reaction, Glu78 functions as a nucleophile ...