Browsing Faculty of Health Sciences (FHS) by Author "Naiki, M"
Now showing items 1-10 of 10
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Binding of Clostridium botulinum neurotoxin to gangliosides
Ochanda James O.; Syuto, B; Ohishi, I; Naiki, M; Kubo, S (1986)The binding characteristics of Clostridium botulinum neurotoxins of types B, C1, and F to gangliosides was studied by thin layer chromatography plate and microtiter plate methods at low (10 mM NaCl in 10 mM Tris-HCl buffer, ... -
Electrically stimulated fusion of human UC 729-6 cell line potentially useful for generating antibody-secreting human-human hybridomas
Gathuru, JK; Miyoshi, I; Naiki, M (University of NairobiSchool of Public Health, University of Nairobi, 1987-04)No abstract available. -
Elevation of Heterophilic antibodies to rabbit erythrocytes in human pathologic sera: quantitative studies by Elisa using a Glycosphingolipid antigen
Suzuki, E; Mukuria, J C; Naiki, M; Kurosawa, T; Hanaue, J (College of Health Sciences, University of Nairobi, 1987)Human heterophilic hemagglutinin to rabbit erythrocytes was quantitatively determined by the hemagglutination test and enzyme linked immunosorbent assay (ELISA) using rabbit erythrocyte pentaglycosyl ceramide (CPH), Gal ... -
Evidence for a free N-acetylneuraminic acid-hydroxylating enzyme in pig mandibular gland soluble fraction
Mukuria, C J; Mwangi, W D; Noguchi, A; Waiyaki, G P; Asano, T; Naiki, M (College of Health Sciences, University of Nairobi, 1995-01-15)The activity of a free N-acetylneuraminic acid (Neu5Ac)-hydroxylating enzyme which converted Neu5Ac into N-glycolyl-neuraminic acid (Neu5Gc) was demonstrated in the soluble fraction of pig mandibular gland. The hydroxylation ... -
Failure of human immunoresponse to N-glycolylneuraminic acid epitope contained in recombinant human erythropoietin
Noguchi, A; Mukuria, C J; Suzuki, E; Naiki, M (College of Health Sciences, University of Nairobi, 1996)Recombinant human erythropoietin (rHUEPO) was produced by Chinese hamster ovary cells and commercially distributed to hospitals by two pharmaceutical companies in Japan ('ESPO' by Kirin Brewery Co. Ltd., and Sankyo Co. ... -
Immunogenicity of N-Glycolylneuraminic Acid-Containing Carbohydrate Chains of Recombinant Human Erythropoietin Expressed in Chinese Hamster Ovary Cells
Noguchi, A; Mukuria, J C; Suzuki, E; Naiki, M (College of Health Sciences, University of Nairobi, 1995)Recombinant human erythropoietin (EPO) produced by Chinese hamster ovary cells and distributed by two different pharmaceutical companies were confirmed to contain about 1% N-glycolylneuraminic acid (Neu5Gc) in total sialic ... -
In vitro immunization of rat spleen lymphocytes with Forssman glycosphingolipid antigen and the generation of a monoclonal antibody
Gathuru, JK; Miyoshi, I; Naiki, M (University of NairobiSchool of Public Health, University of Nairobi, 1991-03)A procedure for in vitro immunization of splenic lymphocytes with a glycolipid antigen is described. Culture medium supernatant of ConA- and PHA-stimulated spleen cells and that of Con A-stimulated human Jurkat T cell line ... -
Potential use of specific human and chicken antibodies for detection of Hanganutziu-Deicher antigen(s) in sera of cancer patients
Mukuria, J C; Noguchi, A; Suzuki, E; Naiki, M (College of Health Sciences, University of Nairobi, 1994-10)A human Hanganutziu-Deicher (HD) antibody and a chicken anti-N-glycolyneuroaminyllactosylceramide (HD3) antibody were compared in their reaction against HD antigen-active ganglioside (HD3) and a glycoprotein (GP) by ... -
A specific enzyme-linked immunosorbent assay (ELISA) procedure for detection of heterophile Hanganutziu and Deicher (HD) antibodies
Mukuria, J C; Naiki, M; Hashimoto, M; Kato, S (College of Health Sciences, University of Nairobi, 1986-02-12)A specific, relatively sensitive, quantitative and standardized enzyme-linked immunosorbent assay (ELISA) procedure was developed for the detection of heterophile Hanganutziu and Deicher (HD) antibodies which are occasionally ... -
Use of biotinylated antibody for the assay of Hanganutziu-Deicher antibodies and antigens in fluids and tissues from cancer patients
Gathuru, JK; Higashi, H; Kato, S; Usuba, O; Naiki, M (University of NairobiSchool of Public Health, University of Nairobi, 1989-04)An improved enzyme-linked immunosorbent assay (ELISA) for detection of heterophile Hanganutziu-Deicher (HD) antibodies and antigens, which are frequently detected in sera and/or cancerous tissues from patients with various ...