Browsing Faculty of Health Sciences (FHS) by Subject "PCR"

Now showing items 1-4 of 4

    • Identification of DNA markers specific to medium-sized chromosomes 4 of Trypanosoma congolense 

      Apopa, P.L; Majiwa, A.O; Kinyanjui, P.W (Intemational Livestock Research InstituteDepartment of Biochemistry, University of Nairobi, 2004)
      Genomic DNA from T. congolense clone I~1180 was purified and resolved by pulsed field gradient gel electrophoresis (PFGE) technique to separate the genome into chromosome-sized DNA molecules. PFGE conditions most ideal for ...

    • Improved template DNA preparation procedure for detection of Mycobacterium avium subsp. paratuberculosis in milk by PCR 

      Gao, Anli; Mutharia, Lucy; Raymond, Melinda (ElsevierDepartment of Molecular and Cellular Biology (Mutharia, Raymond), University of Guelph, Guelph, ON, Canad, 2007)
      Factors affecting the detection of Mycobacterium avium subsp. paratuberculosis (MAP) by PCR in raw milk and their interactions were investigated. Three day old bulk tank raw milk (50 ml) samples were seeded with MAP at a ...

    • Novel thymidylate synthase enhancer region alleles in African populations 

      McLeod Howard L.; Ofori-Adjei David.; Indalo Anne; Githang'a Jessie N.; Ameyaw Margaret M.; Marsh Sharon. (Department of Clinical Medicine and Therapeutics, 2000-12)
      Thymidylate synthase (TS) regulates the production of DNA synthesis precursors and is an important target of cancer chemotherapy. A polymorphic tandem repeat sequence in the enhancer region of the TS promoter was previously ...

    • Trypanosoma (nannomonas) congolense 

      Kinyanjui, PW; Majiwa, PA; Apopa, PL (The Kenya Veterinarian, 2004)
      Pulsed field gradient gel electrophoresis was used to separate chromosomes of parasitic protozoan Trypanosome congolense clone IL1180. Total trypanosome DNA was isolated and resolved into 18 chromosomes by pulsed-field ...