dc.contributor.author | Waruk, JL | |
dc.contributor.author | Machuki, Z | |
dc.contributor.author | Mesa, C | |
dc.contributor.author | Juno, JA | |
dc.contributor.author | Anzala, O | |
dc.contributor.author | Sharma, M | |
dc.contributor.author | Ball, TB | |
dc.contributor.author | Oyugi, J | |
dc.contributor.author | Kiazyk, S | |
dc.date.accessioned | 2015-06-17T05:43:01Z | |
dc.date.available | 2015-06-17T05:43:01Z | |
dc.date.issued | 2015-05-22 | |
dc.identifier.citation | Tuberculosis (Edinb). 2015 May 22. pii: S1472-9792(14)20675-5. | en_US |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/26073895 | |
dc.identifier.uri | http://hdl.handle.net/11295/84961 | |
dc.description.abstract | Mycobacterium tuberculosis (Mtb) infects nearly 2 million people annually and is the most common cause of death in HIV-infected individuals. Tuberculosis (TB) diagnostics cater to HIV-uninfected individuals in non-endemic countries, are expensive, slow, and lack sensitivity for those most affected. Patterns of soluble immune markers from Mtb-stimulated immune cells are not well defined in HIV co-infection. We assessed immune differences between HIV-infected and HIV-uninfected individuals with active TB utilizing IFNγ-based QuantiFERON®-TB Gold In-Tube (QFT) testing in Nairobi, Kenya. Excess QFT supernatants were used to measure cytokine and chemokine responses by a 17-plex bead array. Mtb/HIV co-infected participants were significantly less likely to be QFT+ (47.2% versus 84.2% in the HIV-uninfected group), and demonstrated lower expression of all cytokines except for IFNα2. Receiver operator characteristic analyses identified IL-1α as a potential marker of co-infection. Among HIV-infected individuals, CD4+ T cell count correlated weakly with the expression of several analytes. Co-expression analysis highlighted differences in immune profiles between the groups. These data suggest that there is a unique and detectable Mtb-specific immune response in co-infection. A better understanding of Mtb immunology can translate into much needed immunodiagnostics with enhanced sensitivity in HIV-infected individuals, facilitating their opportunity to obtain live-saving treatment. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.subject | Tuberculosis; HIV; Co-infection; Cytokine; Chemokine; Interferon gamma release assay; ROC curve analysis | en_US |
dc.title | Cytokine and chemokine expression profiles in response to Mycobacterium tuberculosis stimulation are altered in HIV-infected compared to HIV-uninfected subjects with active tuberculosis. | en_US |
dc.type | Article | en_US |
dc.type.material | en | en_US |