Standardization of cytokine flow cytometry assays
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Date
2005Author
Maecker, Holden T
Rinfret, Aline
D'Souza, Patricia
Darden, Janice
Roig, Eva
Landry, Claire
Hayes, Peter
Birungi, Josephine
Anzala, Omu
Garcia, Miguel
Harari, Alexandre
Frank, Ian
Baydo, Ruth
Baker, Megan
Holbrook, Jennifer
Ottinger, Janet
Lamoreaux, Laurie
Epling, C Lorrie
Sinclair, Elizabeth
Suni, Maria A
Punt, Kara
Calarota, Sandra
El-Bahi, Sophia
Alter, Gailet
Maila, Hazel
Kuta, Ellen
Cox, Josephine
Gray, Clive
Altfeld, Marcus
Nougarede, Nolwenn
Boyer, Jean
Tussey, Lynda
Tobery, Timothy
Bredt, Barry
Roederer, Mario
Koup, Richard
Maino, Vernon C
Weinhold, Kent
Pantaleo, Giuseppe
Gilmour, Jill
Horton, Helen
Sekaly, Rafick P
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Background: Cytokine flow cytometry (CFC) or intracellular cytokine staining (ICS) can
quantitate antigen-specific T cell responses in settings such as experimental vaccination.
Standardization of ICS among laboratories performing vaccine studies would provide a commonplatform by which to compare the immunogenicity of different vaccine candidates across multiple
international organizations conducting clinical trials. As such, a study was carried out among several
laboratories involved in HIV clinical trials, to define the inter-lab precision of ICS using various
sample types, and using a common protocol for each experiment (see additional files online).
Subject
ImmunologyCollections
- Faculty of Health Sciences (FHS) [10377]