Quantitative analysis of quaternary ammonium antiseptics using thin-layer densitometry
Date
1994Author
Paesen, J.
Quintens, I.
Thoithi, G.
Roets, E.
Reybrouck, G.
Hoogmartens, J.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A thin-layer chromatography method for quantitative analysis of quaternary ammonium antiseptics is described.
Silanized silica gel was used as the stationary phase. The moille phase consisted of mcthal101-15rc (rn/v) sodium acetate solution-acetone (65:35:20). The method is able to separate the chain homologues of benzalkonium chloride, cetylpyridinium chloride and cetrimide. Detection was performed using a colour reaction with potassium triiodide solution. The different homologues were quantified using UV densitometry at 400 nm. A number of commercial samples was analysed using this method. From the results it appears that it is worthwhile to have a limit test for the composition of quaternary ammonium antiseptics in pharmacopoeial monograph, the more so as the antibacterial activity depends on it.
URI
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10643http://www.ncbi.nlm.nih.gov/pubmed/7921189
Citation
Journal of Chromatography A, 677 (1994) 377-384Publisher
Cathollc University of Leiuven, Laboratory for Pharmaceutical Chemistry; Faculty of Pharmaceutical Sciences. School of Public Health, Faculty of Medicine,
Collections
- Faculty of Health Sciences (FHS) [10387]