Serological Evidence of Exposure to Onyong-nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya
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Date
2022-06Author
Masika, Moses M
Korhonen, Essi M
Smura, Teemu
Uusitalo, Ruut
Ogola, Joseph
Mwaengo, Dufton
Jääskeläinen, Anne J
Alburkat, Hussein
Gwon, Yong-Dae
Evander, Magnus
Anzala, Omu
Vapalahti, Olli
Huhtamo, Eili
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ArticleLanguage
enMetadata
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Several alphaviruses, such as chikungunya (CHIKV) and Onyong-nyong (ONNV), are endemic in Kenya and often cause outbreaks in different parts of the country. We assessed the seroprevalence of alphaviruses in patients with acute febrile illness in two geographically distant areas in Kenya with no previous record of alphavirus outbreaks. Blood samples were collected from febrile patients in health facilities located in the rural Taita-Taveta County in 2016 and urban Kibera informal settlement in Nairobi in 2017 and tested for CHIKV IgG and IgM antibodies using an in-house immunofluorescence assay (IFA) and a commercial ELISA test, respectively. A subset of CHIKV IgG or IgM antibody-positive samples were further analyzed using plaque reduction neutralization tests (PRNT) for CHIKV, ONNV, and Sindbis virus. Out of 537 patients, 4 (0.7%) and 28 (5.2%) had alphavirus IgM and IgG antibodies, respectively, confirmed on PRNT. We show evidence of previous and current exposure to alphaviruses based on serological testing in areas with no recorded history of outbreaks.
Citation
Masika MM, Korhonen EM, Smura T, Uusitalo R, Ogola J, Mwaengo D, Jääskeläinen AJ, Alburkat H, Gwon YD, Evander M, Anzala O, Vapalahti O, Huhtamo E. Serological Evidence of Exposure to Onyong-Nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-Taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya. Viruses. 2022 Jun 13;14(6):1286. doi: 10.3390/v14061286. PMID: 35746757; PMCID: PMC9230508.Publisher
University of Nairobi
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Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
- Faculty of Health Sciences (FHS) [10387]
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