Construction of an infectious HIV type 1 molecular clone from an African patient with a subtype D/C Recombinant Virus.
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Date
2004Author
Shi, B
Philpott, SM
Weiser, B
Kuiken, C
Brunner, C
Fang, G
Fowke, KR
Plummer, FA
Rowland-Jones, S
Bwayo, JJ
Anzala Aggrey O.
Kimani, J
Kaul, R
Burger, H
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The majority of HIV-1 infections worldwide occur in Africa, where subtype B viruses are rare and intersubtype recombinants are common. Pathogenesis and vaccine studies need to focus on viruses derived from African patients, and infectious HIV-1 molecular clones can be useful tools. To clone non-B subtypes and recombinant viruses from patients, we cultivated HIV-1 from the plasma of a Kenyan long-term survivor. Viral DNA was cloned into a plasmid, which was transfected into COS cells; progeny virus was propagated in PBMCs. Sequence analyses revealed that both the patient's plasma HIV-1 RNA and the cloned DNA genomes were recombinants between subtypes D and C; subtype C sequences comprised the nef and LTR regions. The cloned virus used the CCR5 coreceptor and did not form syncytia in vitro. This infectious HIV-1 subtype D/C recombinant molecular clone obtained from a Kenyan long-term survivor promises to be useful to study pathogenesis and vaccine design
URI
http://www.ncbi.nlm.nih.gov/pubmed/15585089http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/17125
Citation
AIDS Res Hum Retroviruses. 2004 Sep;20(9):1015-8Publisher
Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA
Collections
- Faculty of Health Sciences (FHS) [10377]