dc.contributor.author | Shi, B | |
dc.contributor.author | Philpott, SM | |
dc.contributor.author | Weiser, B | |
dc.contributor.author | Kuiken, C | |
dc.contributor.author | Brunner, C | |
dc.contributor.author | Fang, G | |
dc.contributor.author | Fowke, KR | |
dc.contributor.author | Plummer, FA | |
dc.contributor.author | Rowland-Jones, S | |
dc.contributor.author | Bwayo, JJ | |
dc.contributor.author | Anzala Aggrey O. | |
dc.contributor.author | Kimani, J | |
dc.contributor.author | Kaul, R | |
dc.contributor.author | Burger, H | |
dc.date.accessioned | 2013-04-26T12:12:23Z | |
dc.date.available | 2013-04-26T12:12:23Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | AIDS Res Hum Retroviruses. 2004 Sep;20(9):1015-8 | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/15585089 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/17125 | |
dc.description.abstract | The majority of HIV-1 infections worldwide occur in Africa, where subtype B viruses are rare and intersubtype recombinants are common. Pathogenesis and vaccine studies need to focus on viruses derived from African patients, and infectious HIV-1 molecular clones can be useful tools. To clone non-B subtypes and recombinant viruses from patients, we cultivated HIV-1 from the plasma of a Kenyan long-term survivor. Viral DNA was cloned into a plasmid, which was transfected into COS cells; progeny virus was propagated in PBMCs. Sequence analyses revealed that both the patient's plasma HIV-1 RNA and the cloned DNA genomes were recombinants between subtypes D and C; subtype C sequences comprised the nef and LTR regions. The cloned virus used the CCR5 coreceptor and did not form syncytia in vitro. This infectious HIV-1 subtype D/C recombinant molecular clone obtained from a Kenyan long-term survivor promises to be useful to study pathogenesis and vaccine design | en |
dc.language.iso | en | en |
dc.title | Construction of an infectious HIV type 1 molecular clone from an African patient with a subtype D/C Recombinant Virus. | en |
dc.type | Article | en |
local.publisher | Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA | en |