Pooled HIV-1 RNA viral load testing for detection of antiretroviral treatment failure in Kenyan children
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Date
2013-03Author
Chohan, B. H.
Tapia, K.
Merkel, M.
Kariuki, A. C.
Khasimwa, B.
Olago, A.
Gichohi, R.
Obimbo, E. M.
Wamalwa, D. C.
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BACKGROUND:: Pooled viral load (VL) testing with two different testing strategies was evaluated as a potential cost-saving method to monitor antiretroviral therapy (ART) in HIV-infected children receiving ART in a resource-limited setting. METHODS:: Archived samples collected from 250 HIV-1 infected children on first-line ART at various time-points post-ART initiation were evaluated for pooled VL testing using a minipool+algorithm strategy. Additionally, samples collected in real-time from 125 children on ART were assessed for virologic failure using a minipool strategy for pooled viral load testing. Virologic failure was determined as HIV-1 RNA viral loads >1500 copies/ml. RESULTS:: Minipool+algorithm strategy for pooled VL testing of archived samples had estimated viral failure of 13.6%, with a relative efficiency (RE) of 23.6% (95% CI; 18.5, 29.4), and negative predictive value (NPV) of 88%. This testing strategy would have resulted in 24% fewer assays needed, for a cost savings of $1,180 per 100 samples. The minipool strategy for pooled viral load testing of samples obtained in real-time yielded an estimated 23.2% of samples with viral failure and a RE of 8.0 % (95% CI; 3.9, 14.2); however had a minipool+algorithm pooling strategy been used the RE would increase to 20%. CONCLUSIONS:: The minipool+algorithm strategy for pooled VL testing to detect virologic failure in HIV-1 infected children on ART was determined to be relatively efficient in detecting virologic failure, had high NPV, with substantial cost savings. Pooling strategies may be important components of cost-effect strategies to reduce rates of viral failure and resistance, thus improving clinical outcomes.
URI
http://www.ncbi.nlm.nih.gov/pubmed/23542638http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/23242
Citation
J Acquir Immune Defic Syndr.Publisher
Departments of Medical Microbiology, University of Nairobi, Nairobi, Kenya Department of Pediatrics and Child Health, University of Nairobi, Nairobi, Kenya
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- Faculty of Health Sciences (FHS) [10378]