dc.contributor.author | Chohan, B. H. | |
dc.contributor.author | Tapia, K. | |
dc.contributor.author | Merkel, M. | |
dc.contributor.author | Kariuki, A. C. | |
dc.contributor.author | Khasimwa, B. | |
dc.contributor.author | Olago, A. | |
dc.contributor.author | Gichohi, R. | |
dc.contributor.author | Obimbo, E. M. | |
dc.contributor.author | Wamalwa, D. C. | |
dc.date.accessioned | 2013-05-15T12:49:02Z | |
dc.date.available | 2013-05-15T12:49:02Z | |
dc.date.issued | 2013-03 | |
dc.identifier.citation | J Acquir Immune Defic Syndr. | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/23542638 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/23242 | |
dc.description.abstract | BACKGROUND:: Pooled viral load (VL) testing with two different testing strategies was evaluated as a potential cost-saving method to monitor antiretroviral therapy (ART) in HIV-infected children receiving ART in a resource-limited setting. METHODS:: Archived samples collected from 250 HIV-1 infected children on first-line ART at various time-points post-ART initiation were evaluated for pooled VL testing using a minipool+algorithm strategy. Additionally, samples collected in real-time from 125 children on ART were assessed for virologic failure using a minipool strategy for pooled viral load testing. Virologic failure was determined as HIV-1 RNA viral loads >1500 copies/ml. RESULTS:: Minipool+algorithm strategy for pooled VL testing of archived samples had estimated viral failure of 13.6%, with a relative efficiency (RE) of 23.6% (95% CI; 18.5, 29.4), and negative predictive value (NPV) of 88%. This testing strategy would have resulted in 24% fewer assays needed, for a cost savings of $1,180 per 100 samples. The minipool strategy for pooled viral load testing of samples obtained in real-time yielded an estimated 23.2% of samples with viral failure and a RE of 8.0 % (95% CI; 3.9, 14.2); however had a minipool+algorithm pooling strategy been used the RE would increase to 20%. CONCLUSIONS:: The minipool+algorithm strategy for pooled VL testing to detect virologic failure in HIV-1 infected children on ART was determined to be relatively efficient in detecting virologic failure, had high NPV, with substantial cost savings. Pooling strategies may be important components of cost-effect strategies to reduce rates of viral failure and resistance, thus improving clinical outcomes. | en |
dc.relation.ispartofseries | Mar 28. [Epub ahead of print]; | |
dc.title | Pooled HIV-1 RNA viral load testing for detection of antiretroviral treatment failure in Kenyan children | en |
local.publisher | Departments of Medical Microbiology, University of Nairobi, Nairobi, Kenya | en |
local.publisher | Department of Pediatrics and Child Health, University of Nairobi, Nairobi, Kenya | en |