Proline transport by tsetse fly Glossina morsitans flight muscle mitochondria.
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Date
1992-07Author
Njagi, E N
Olembo, N K
Pearson, D J
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Proline accumulation by tsetse fly Glossina morsitans flight muscle mitochondria was studied in vitro by the swelling technique and direct measurement of (U-14C) proline. 2. Proline transport was inhibited by the uncharged liposoluble -SH reagent, N-ethylmaleimide but not by ionic reagent, mersalyl, suggesting that the -SH groups involved in the transport of proline are located in a hydrophobic part of the membrane or on the matrix side of the membrane. 3. The kinetic study of proline accumulation revealed saturation kinetics and a high temperature dependence. It gave a Km of 85 microM and a Vmax of 962 pmol/min/mg protein and an activation energy (Ea) of 11 kcal/mol. 4. Certain other amino acids (L-valine, L-alanine, L-methionine, L-phenylalanine, L-tryptophan and L-hydroxyproline) significantly stimulated proline uptake. 5. These observations indicate that tsetse fly Glossina morsitans flight muscle mitochondria contain a proline transport mechanism.
URI
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/30902http://www.ncbi.nlm.nih.gov/pubmed/1499295
Citation
Comp Biochem Physiol B. 1992 Jul;102(3):579-84Publisher
University of Nairobi, Department of Biochemistry, University of Nairobi, Kenya.
Collections
- Faculty of Health Sciences (FHS) [10387]