dc.contributor.author | Njagi, E N | |
dc.contributor.author | Olembo, N K | |
dc.contributor.author | Pearson, D J | |
dc.date.accessioned | 2013-06-10T13:13:12Z | |
dc.date.available | 2013-06-10T13:13:12Z | |
dc.date.issued | 1992-07 | |
dc.identifier.citation | Comp Biochem Physiol B. 1992 Jul;102(3):579-84 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/30902 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/1499295 | |
dc.description.abstract | Proline accumulation by tsetse fly Glossina morsitans flight muscle mitochondria was studied in vitro by the swelling technique and direct measurement of (U-14C) proline. 2. Proline transport was inhibited by the uncharged liposoluble -SH reagent, N-ethylmaleimide but not by ionic reagent, mersalyl, suggesting that the -SH groups involved in the transport of proline are located in a hydrophobic part of the membrane or on the matrix side of the membrane. 3. The kinetic study of proline accumulation revealed saturation kinetics and a high temperature dependence. It gave a Km of 85 microM and a Vmax of 962 pmol/min/mg protein and an activation energy (Ea) of 11 kcal/mol. 4. Certain other amino acids (L-valine, L-alanine, L-methionine, L-phenylalanine, L-tryptophan and L-hydroxyproline) significantly stimulated proline uptake. 5. These observations indicate that tsetse fly Glossina morsitans flight muscle mitochondria contain a proline transport mechanism. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi, | en |
dc.title | Proline transport by tsetse fly Glossina morsitans flight muscle mitochondria. | en |
dc.type | Article | en |
local.publisher | Department of Biochemistry, University of Nairobi, Kenya. | en |