Polymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo hemorrhagic fever virus in the United Arab Emirates.
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Date
1996-08Author
Schwarz, TF
Nsanze, H
Longson, M
Nitschko, H
Gilch, S
Shurie, H
Ameen, A
Zahir, AR
Acharya, UG
Jager, G
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Viral hemorrhagic fever has re-emerged in the United Arab Emirates (UAE) since November 1993. Genomic RNA of Crimean-Congo hemorrhagic virus (C-CHFV) was detected by a newly developed, nested reverse transcriptase polymerase chain reaction (RT-PCR) in the sera of four (25.0%) of 16 suspected cases of viral hemorrhagic fever. The RT-PCR was based on oligonucleotide primers deducted from the small RNA segment encoding the nucleoprotein of the virus. By comparison with a nucleotide sequence of a C-CHFV isolate from a Chinese sheep, a divergence of 10.0-11.8% was detected in the C-CHFV variants causing the UAE outbreak. In the four positive sera, three phylogenetically distinct C-CHFV variants were amplified and confirmed by direct sequencing of the PCR fragments. These C-CHFV sequences were obtained directly from sera of infected humans without prior propagation in cell culture. The RT-PCR allows rapid detection of genomic C-CHFV RNA in clinical specimens and study of the molecular epidemiology of this infection.
URI
http://www.ncbi.nlm.nih.gov/pubmed/8780459http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31001
Citation
Am J Trop Med Hyg. 1996 Aug;55(2):190-6.Publisher
University of Nairobi. Department of Medicine
Collections
- Faculty of Health Sciences (FHS) [10387]