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dc.contributor.authorSchwarz, TF
dc.contributor.authorNsanze, H
dc.contributor.authorLongson, M
dc.contributor.authorNitschko, H
dc.contributor.authorGilch, S
dc.contributor.authorShurie, H
dc.contributor.authorAmeen, A
dc.contributor.authorZahir, AR
dc.contributor.authorAcharya, UG
dc.contributor.authorJager, G
dc.date.accessioned2013-06-10T14:39:17Z
dc.date.available2013-06-10T14:39:17Z
dc.date.issued1996-08
dc.identifier.citationAm J Trop Med Hyg. 1996 Aug;55(2):190-6.en
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/8780459
dc.identifier.urihttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31001
dc.description.abstractViral hemorrhagic fever has re-emerged in the United Arab Emirates (UAE) since November 1993. Genomic RNA of Crimean-Congo hemorrhagic virus (C-CHFV) was detected by a newly developed, nested reverse transcriptase polymerase chain reaction (RT-PCR) in the sera of four (25.0%) of 16 suspected cases of viral hemorrhagic fever. The RT-PCR was based on oligonucleotide primers deducted from the small RNA segment encoding the nucleoprotein of the virus. By comparison with a nucleotide sequence of a C-CHFV isolate from a Chinese sheep, a divergence of 10.0-11.8% was detected in the C-CHFV variants causing the UAE outbreak. In the four positive sera, three phylogenetically distinct C-CHFV variants were amplified and confirmed by direct sequencing of the PCR fragments. These C-CHFV sequences were obtained directly from sera of infected humans without prior propagation in cell culture. The RT-PCR allows rapid detection of genomic C-CHFV RNA in clinical specimens and study of the molecular epidemiology of this infection.en
dc.language.isoenen
dc.publisherUniversity of Nairobi.en
dc.titlePolymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo hemorrhagic fever virus in the United Arab Emirates.en
dc.typeArticleen
local.publisherDepartment of Medicineen


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