dc.contributor.author | Schwarz, TF | |
dc.contributor.author | Nsanze, H | |
dc.contributor.author | Longson, M | |
dc.contributor.author | Nitschko, H | |
dc.contributor.author | Gilch, S | |
dc.contributor.author | Shurie, H | |
dc.contributor.author | Ameen, A | |
dc.contributor.author | Zahir, AR | |
dc.contributor.author | Acharya, UG | |
dc.contributor.author | Jager, G | |
dc.date.accessioned | 2013-06-10T14:39:17Z | |
dc.date.available | 2013-06-10T14:39:17Z | |
dc.date.issued | 1996-08 | |
dc.identifier.citation | Am J Trop Med Hyg. 1996 Aug;55(2):190-6. | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/8780459 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31001 | |
dc.description.abstract | Viral hemorrhagic fever has re-emerged in the United Arab Emirates (UAE) since November 1993. Genomic RNA of Crimean-Congo hemorrhagic virus (C-CHFV) was detected by a newly developed, nested reverse transcriptase polymerase chain reaction (RT-PCR) in the sera of four (25.0%) of 16 suspected cases of viral hemorrhagic fever. The RT-PCR was based on oligonucleotide primers deducted from the small RNA segment encoding the nucleoprotein of the virus. By comparison with a nucleotide sequence of a C-CHFV isolate from a Chinese sheep, a divergence of 10.0-11.8% was detected in the C-CHFV variants causing the UAE outbreak. In the four positive sera, three phylogenetically distinct C-CHFV variants were amplified and confirmed by direct sequencing of the PCR fragments. These C-CHFV sequences were obtained directly from sera of infected humans without prior propagation in cell culture. The RT-PCR allows rapid detection of genomic C-CHFV RNA in clinical specimens and study of the molecular epidemiology of this infection. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi. | en |
dc.title | Polymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo hemorrhagic fever virus in the United Arab Emirates. | en |
dc.type | Article | en |
local.publisher | Department of Medicine | en |