Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa
Date
2003-12Author
Ochong, E
Nzila, A
Kimani, S
Kokwaro, G
Mutabingwa, T
Watkins, W
Marsh, K
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation
URI
http://www.ncbi.nlm.nih.gov/pubmed/14675491http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31140
Citation
Malar J. 2003 Dec 15;2(1):46Publisher
University of Nairobi College of Health Sciences
Collections
- Faculty of Health Sciences (FHS) [10378]