Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa

dc.contributor.author	Ochong, E
dc.contributor.author	Nzila, A
dc.contributor.author	Kimani, S
dc.contributor.author	Kokwaro, G
dc.contributor.author	Mutabingwa, T
dc.contributor.author	Watkins, W
dc.contributor.author	Marsh, K
dc.date.accessioned	2013-06-11T07:02:26Z
dc.date.available	2013-06-11T07:02:26Z
dc.date.issued	2003-12
dc.identifier.citation	Malar J. 2003 Dec 15;2(1):46	en
dc.identifier.uri	http://www.ncbi.nlm.nih.gov/pubmed/14675491
dc.identifier.uri	http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31140
dc.description.abstract	The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation	en
dc.language.iso	en	en
dc.publisher	University of Nairobi	en
dc.title	Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa	en
dc.type	Article	en
local.publisher	College of Health Sciences	en