dc.contributor.author | McKinnon, LR | |
dc.contributor.author | Ball, TB | |
dc.contributor.author | Wachihi, C, | |
dc.contributor.author | Chinga, N | |
dc.contributor.author | Maingi, A | |
dc.contributor.author | Luo, M | |
dc.contributor.author | Fowke, K R | |
dc.contributor.author | Plummer, FA | |
dc.date.accessioned | 2013-06-27T13:55:28Z | |
dc.date.available | 2013-06-27T13:55:28Z | |
dc.date.issued | 2008-10 | |
dc.identifier.citation | J Acquir Immune Defic Syndr. 2008 Oct 1;49(2):123-7. doi: 10.1097/QAI.0b013e3181869a88. | en |
dc.identifier.uri | http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/18769359 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/41256 | |
dc.description.abstract | HIV vaccine design and evaluation require a better understanding of protective immune responses. HIV-specific CD8+ T-cell responses have been characterized extensively using interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot (ELISPOT) assays, which do not always correlate with control of viral replication or disease progression. Alternative aspects of CD8+ T-cell responses, in particular those associated with a central memory (Tcm) phenotype, may be more protective against disease progression. To determine the extent that the breadth and specificity of HIV-specific CD8+ T-cell responses differ based on immunological readout, we screened in HIV-infected Kenyan sex workers for responses to HIV Env using IFN-gamma ELISPOT and 6-day carboxyfluorescein succinimidyl ester-based proliferation assays. This comparison revealed substantial differences in the epitopes recognized when the assay readout was IFN-gamma versus proliferation. Although 24 and 41 IFN-gamma and proliferative responses were identified, overlapping specificity was observed for only 5 responses. Breadth also differed between assays in several patients. Env-specific IFN-gamma breadth was found to correlate inversely with CD4 count (r = -0.66, P = 0.005), although this was not the case for proliferation. These data suggest that efforts to define HIV-specific CD8+ T-cell responses may need to be revisited using additional immunological readouts. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Substantial Intrapatient Differences In The Breadth And Specificity Of Hiv-specific Cd8+ T-cell Interferon-gamma And Proliferation Responses. | en |
dc.type | Article | en |
local.publisher | Department of Medical Microbiology | en |
local.publisher | Department of Medical Microbiology | en |