Functional HIV-1 specific IgA antibodies in HIV-1 exposed, persistently IgG seronegative female sex workers
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Date
2001-11Author
Broliden, Kristina
Hinkula, Jorma
Devito, Claudia
Kiama, Peter
Kimani, Joshua
Trabbatoni, Daria
Bwayo, Job J.
Clerici, Mario
Plummer, Francis
Kaul, Rupert
Type
ArticleLanguage
enMetadata
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Although HIV-specific cellular immune responses are found in a number of HIV highly-exposed, persistently seronegative (HEPS) cohorts, late seroconversion can occur despite pre-existing cytotoxic T lymphocytes (CTL), suggesting that a protective HIV vaccine may need to induce a broader range of HIV-specific immune responses. Low levels of HIV-specific IgA have been found in the genital tract and plasma of the majority of Nairobi HEPS sex workers and appeared to be independent of HIV-specific cellular responses. IgA purified from genital tract, saliva and plasma of most HEPS sex workers were able to neutralize infection of PBMC by a primary (NSI) clade B HIV isolate, as well as viral isolates from clades A and D, which predominate in Kenya. In addition, these IgA were able to inhibit transcytosis of infective HIV virions across a transwell model of the human mucosal epithelium in an HIV-specific manner. Preliminary work in other HEPS cohorts has suggested the recognition of different gp41 epitopes in HEPS and HIV-infected subjects. Although present at low levels, these IgA demonstrated cross-clade neutralizing activity and were able to inhibit HIV mucosal transcytosis, suggesting an important functional role in protection against HIV infection.
URI
http://www.ncbi.nlm.nih.gov/pubmed/11595287http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/41616
Citation
Immunology LettersPublisher
Department of Medical Microbiology, University of Nairobi, P.O. Box 19676, Nairobi, Kenya Department of Clinical Virology, F68, Karolinska Institute, Huddinge University Hospital, S-141 86 Stockholm, Sweden Department of Clinical Virology, Swedish Institute for Infectious Disease Control, Microbiology and Tumor Biology Center, Karolinska Institute, S-105 21 Stockholm, Sweden Chair of Immunology, University of Milan, Milan, Italy Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada MRC Human Immunology Unit, Institute of Molecular Medicine, Oxford, UK
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- Faculty of Health Sciences (FHS) [10387]