An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgM anti-idiotypes directed against anti-HBs molecules.
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Date
1987Author
Irshad, M
Gandhi, BM
Acharya, KS
Joshi, YK
Tandon, BN
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A simple and specific enzyme-linked immunosorbent assay (ELISA) has been developed to detect circulating IgG and IgM anti-idiotypic antibodies directed against anti-HBs molecules using 96-well polyvinyl microtitre plates as the solid phase and HRPO-labelled goat anti-HBs as conjugate. Anti-idiotype reactions were observed in the supernatant portion after precipitation of immune complexes from sera with polyethylene glycol 6000 (PEG). Both IgG and IgM with anti-idiotype activity were detected concurrently in HBsAg-positive sera from HBV-infected patients and asymptomatic HBV carriers. Anti-idiotype activity was absent in HBsAg-negative sera from healthy persons, and in patients with non-A, non-B hepatitis and viral hepatitis A. However, such antibodies could be demonstrated in the sera of two out of eight HBsAg vaccine recipients negative for anti-HBs but in none of 11 recipients positive for anti-HBs after receiving a booster immunising dose of HBsAg vaccine. Those sera showing positive anti-idiotype reactions were free from rheumatoid factor and HBsAg/IgM or HBsAg/IgG complex activity. An analysis of anti-idiotype positive sera for anti-HBs, HBeAg and HBV-specific DNA-polymerase activity demonstrated these markers in 20%, 30% and 60% of cases, respectively. The presence of anti-idiotypic antibodies was presumed to permit a more active multiplication of hepatitis B virus.
URI
http://profiles.uonbi.ac.ke/sacharya/publications/irshad-m-gandhi-bm-acharya-sk-joshi-yk-tandon-bnan-enzyme-linked-immunosorbenthttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/46812
Citation
KIRTDA, DRACHARYAS. 1987. Irshad M, Gandhi BM, Acharya SK, Joshi YK, Tandon BN.An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgM anti-idiotypes directed against anti-HBs molecules.J Immunol Methods. 1987 Feb 11;96(2):211-7..Publisher
Department of Medicine, College of Health Sciences, University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10377]