dc.contributor.author | Irshad, M | |
dc.contributor.author | Gandhi, BM | |
dc.contributor.author | Acharya, KS | |
dc.contributor.author | Joshi, YK | |
dc.contributor.author | Tandon, BN | |
dc.date.accessioned | 2013-07-09T14:14:35Z | |
dc.date.available | 2013-07-09T14:14:35Z | |
dc.date.issued | 1987 | |
dc.identifier.citation | KIRTDA, DRACHARYAS. 1987. Irshad M, Gandhi BM, Acharya SK, Joshi YK, Tandon BN.An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgM anti-idiotypes directed against anti-HBs molecules.J Immunol Methods. 1987 Feb 11;96(2):211-7.. | en |
dc.identifier.uri | http://profiles.uonbi.ac.ke/sacharya/publications/irshad-m-gandhi-bm-acharya-sk-joshi-yk-tandon-bnan-enzyme-linked-immunosorbent | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/46812 | |
dc.description.abstract | A simple and specific enzyme-linked immunosorbent assay (ELISA) has been developed to detect circulating IgG and IgM anti-idiotypic antibodies directed against anti-HBs molecules using 96-well polyvinyl microtitre plates as the solid phase and HRPO-labelled goat anti-HBs as conjugate. Anti-idiotype reactions were observed in the supernatant portion after precipitation of immune complexes from sera with polyethylene glycol 6000 (PEG). Both IgG and IgM with anti-idiotype activity were detected concurrently in HBsAg-positive sera from HBV-infected patients and asymptomatic HBV carriers. Anti-idiotype activity was absent in HBsAg-negative sera from healthy persons, and in patients with non-A, non-B hepatitis and viral hepatitis A. However, such antibodies could be demonstrated in the sera of two out of eight HBsAg vaccine recipients negative for anti-HBs but in none of 11 recipients positive for anti-HBs after receiving a booster immunising dose of HBsAg vaccine. Those sera showing positive anti-idiotype reactions were free from rheumatoid factor and HBsAg/IgM or HBsAg/IgG complex activity. An analysis of anti-idiotype positive sera for anti-HBs, HBeAg and HBV-specific DNA-polymerase activity demonstrated these markers in 20%, 30% and 60% of cases, respectively. The presence of anti-idiotypic antibodies was presumed to permit a more active multiplication of hepatitis B virus. | en |
dc.language.iso | en | en |
dc.title | An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgM anti-idiotypes directed against anti-HBs molecules. | en |
dc.type | Article | en |
local.publisher | Department of Medicine, College of Health Sciences, University of Nairobi | en |