The ATM Tumour Suppressor Gene Is Down-regulated In EBV-associated Nasopharyngeal Carcinoma.
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Date
2009Author
Murray, PG
Woodman, CB
Stankovic, T
Teo, SH
Young, LS
Lim, PV
Arrand, J
Wei, W
Buettner, M
Maina, E
Chukwuma, MB
Dawson, C
Morgan, S
Saleh, A
Starzcynski, J
Fung, M
Yap, LF
Bose, S
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A micro-array analysis using biopsies from patients with EBV-positive undifferentiated nasopharyngeal carcinoma (NPC) and from cancer-free controls revealed down-regulation of tumour suppressor genes (TSG) not previously associated with this disease; one such gene was the ataxia telangiectasia mutated (ATM) gene. Q-PCR confirmed down-regulation of ATM mRNA and ATM protein expression in tumour cells was weak or absent in almost all cases. In NPC cell lines, however, ATM was down-regulated only in the EBV-positive line, C666.1, and in none of five EBV-negative lines. In vitro infection of EBV-negative NPC cell lines with a recombinant EBV was followed by the down-regulation of ATM mRNA and protein, and only EBV-positive cells showed a defective DNA damage response following gamma-irradiation. Our data suggest that loss of ATM function could be an important step in the pathogenesis of NPC, and may have implications for the treatment of this disease.
URI
http://www.ncbi.nlm.nih.gov/pubmed/19142888http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/48483
Citation
J Pathol. 2009 Feb;217(3):345-52. doi: 10.1002/path.2487.Publisher
Department of Biochemistry
Collections
- Faculty of Health Sciences (FHS) [10387]