Browsing Journal Articles by Author "Xuan, Xuenan"
Now showing items 1-18 of 18
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Artesunate, a potential drug for treatment of Babesia infection
Goo, Youn-Kyoung; Terkawi, M. Alaa; Jia, Honglin; Aboge, G. Oluga; Ooka, Hideo; Nelson, Bryce; Kim, Suk; Sunaga, Fujiko; Namikawa, Kazuhiko; Igarashi, Ikuo; Nishikawa, Yoshifumi; Xuan, Xuenan (2010)The effects of artesunate, a water-soluble artemisinin derivative, against Babesia species, including Babesia bovis, Babesia gibsoni and Babesia microti were studied. Cultures of B. bovis and B. gibsoni were treated with ... -
Babesia gibsoni: Identification of an immunodominant, interspersed repeat antigen
Jia, Honglin; Terkawi, M. Alaa; Aboge, Gabriel Oluga; Goo, Youn-Kyoung; Zhou, Jinlin; Lee, Eung-Goo; Nishikawa, Yoshifumi; Igarashi, Ikuo; Fujisaki, Kozo; Xuan, Xuenan (2008)In this report, an immunodominant antigen called BgIRA from Babesia gibsoni is identified and described. A highly repetitive antigen was screened from a cDNA library. The genomic BgIRA gene exists as single cope gene and ... -
Babesia gibsoni: Serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant BgTRAP
Goo, Youn-Kyoung; Jia, Honglin; Aboge, G O; Terkawi, M A; Kuriki, Ken; Nakamura, Chinatsu; Kumagai, Akiko; Zhou, Lin Jin; Lee, Eung-goo; Nishikawa, Yoshifumi; Igarashi, Ikuo; Fujisaki, Kozo; Xuan, Xuenan (Department of Public Health Pharmacology and Toxicology, 2008)The thrombospondin-related adhesive protein of Babesia gibsoni (BgTRAPJ is known as an immunodominant antigen and is, there¬fore, considered as a candidate for the developmentof a diagnostic reagent for canine babesiosis. ... -
Characterization of the Babesia gibsoni 12-kDa protein as a potential antigen for the serodiagnosis
Goo, Youn-Kyoung; Jia, Honglin; Aboge, G. Oluga; Terkawi, M. Alaa; Lee, Eung-goo; Yamagishi, Junya; Nishikawa, Yoshifumi; Jang, Hyung-Kwan; Sunaga, Fujiko; Namikawa, Kazuhiko; Fujisaki, Kozo; Xuan, Xuenan (2009)A novel gene, BgP12, encoding a 12-kDa protein was identified from Babesia gibsoni. The full-length cDNA of BgP12 contains an open reading frame of 378 bp, corresponding to 126 amino acid (aa) residues consisting of a ... -
Cloning, characterization and validation of inosine 5′-monophosphate dehydrogenase of Babesia gibsoni as molecular drug target
Xuan, Xuenan; Nishikawa, Yoshifumi; Igarashi, Ikuo; Suzuki, Hiroshi; Masatani, Tatsunori; Kamyingkird, Ketsarin; Goo, Younkyoung; Li, Yan; Yu, Longzheng; Luo, Yuzi; Zhou, Mo; Terkawi, Mohamad A; Aboge, Gabriel O; Cao, Shinuo (Department of Public Health, Pharmacology and Toxicology, 2013-04)The inosine monophosphate dehydrogenase (IMPDH) enzyme has been characterized and validated as a molecular drug target in other apicomplexans but not in the genus Babesia. Subsequently, we cloned and expressed a Babesia ... -
Cloning, Expression, and Characterization of Babesia gibsoni Dihydrofolate Reductase-Thymidylate Synthase: Inhibitory Effect of Antifolates on Its Catalytic Activity and Parasite Proliferation
Aboge, Gabriel O; Terkawi, Mohamad A; Goo, Youn-Kyoung; Nishikawa, Yoshifumi; Sunaga, Fujiko; Namikawa, Kuzuhiko; Tsuji, Naotoshi; Igarashi, Ikuo; Suzuki, Hiroshi; Fujisaki, Kozo; Xuan, Xuenan (Department of Public Health Pharmacology and Toxicology, 2008)Dihydrofolate reductase-thymidylate synthase (DHFR-TS) is a well-validated antifolate drug target in certain pathogenic apicomplexans, but not in the genus Babesia, including Babesia gibsoni. Therefore, we isolated, cloned, ... -
Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni
Goo, Youn-Kyoung; Lee, Naeun; Terkawi, Mohamad Alaa; Luo, Yuzi; Aboge, Gabriel Oluga; Nishikawa, Yoshifumi; Suzuki, Hiroshi; Kim, Suk; Xuan, Xuenan (2012)We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, ... -
Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay
Narantsatsral, S; Goo, Youn-Kyoung; Battsetseg, B; Myagmarsuren, P; Terkawi, Mohamad Alaa; Soma, Takehisa; Luo, Yuzi; Lia, Yan; Cao, Shinuo; Yu, Longzheng; Kamyingkird, Ketsarin; Aboge, Gabriel Oluga; Nishikawa, Yoshifumi; Xuan, Xuenan (2011)Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of a recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most ... -
Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family
Goo, Youn-Kyoung; Aboge, Gabriel Oluga; Terkawi, M. Alaa; Jia, Honglin; Yamagishi, Junya; Sunaga, Fujiko; Namikawa, Kazuhiko; Cha, Se-Yeoun; Jang, Hyung-Kwan; Kim, Suk; Nishikawa, Yoshifumi; Xuan, Xuenan (2012)We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding ... -
Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen
Terkawi, M. A.; Aboge, G. Oluga; Jia, H; Goo, Y K; Ooka, H; Yamagishi, Junya; Nishikawa, Yoshifumi; Shin-ichiro, Kawazu; Fujisaki, K; Xuan, Xuenan (Department of Public Health, Pharmacology & Toxicology, University of Nairobi, 2008)A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 ... -
Identification of Secreted Antigen 3 from Babesia gibsoni
Jia, Honglin; Terkawi, M. Alaa; Aboge, Gabriel Oluga; Goo, Youn-Kyoung; Ma, Liqing; Zhou, Jinlin; Nishikawa, Yoshifumi; Igarashi, Ikuo; Fujisaki, Kozo; Xuan, Xuenan (Department of Public Health, Pharmacology and Toxicology, 2009)A cDNA expression library of Babesia gibsoni was screened with the serum collected from a dog experimentally infected with B. gibsoni. A novel antigen sharing homology with secreted antigen 1 of B. gibsoni was isolated. ... -
Macrophages Are Critical for Cross-Protective Immunity Conferred by Babesia microti against Babesia rodhaini Infection in Mice
Li, Yan; Terkawi, Mohamad Alaa; Nishikawa, Yoshifumi; Aboge, Gabriel Oluga; Luo, Yuzi; Ooka, Hideo; Goo, Youn-Kyoung; Yu, Longzheng; Cao, Shinuo; Sun, Yongfeng; Yamagishi, Junya; Masatani, Tatsunori; Yokoyama, Naoaki; Igarashi, Ikuo; Xuan, Xuenan (Department of Public Health, Pharmacology and Toxicology, 2012)AlthoughprimaryinfectionofmicewithBabesiamicrotihasbeenshowntoprotectmiceagainstsubsequentlethalinfection by Babesiarodhaini,themechanismbehindthecross-protection is unknown.To unravel lthis mechanism,we investigated the ... -
Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand
Cao, Shinuo; Aboge, Gabriel Oluga; Terkawi, Mohamad Alaa; Yu, Longzheng; Kamyingkird, Ketsarin; Luo, Yuzi; Li, Yan; Goo, Youn-Kyoung; Yamagishi, Junya; Nishikawa, Yoshifumi; Yokoyama, Naoaki; Suzuki, Hiroshi; Igarashi, Ikuo; Maeda, Ryuichiro; Inpankaew, Tawin; Jittapalapong, Sathaporn; Xuan, Xuenan (2012)Although Babesia bovis and Babesia bigemina infections cause economic losses in the cattle industry in northern Thailand, there is inadequate information on Babesia isolates present in the area. Therefore, to determine the ... -
Mycophenolic Acid, Mycophenolate Mofetil, Mizoribine, Ribavirin, and 7-Nitroindole Inhibit Propagation of Babesia Parasites by Targeting Inosine 5'-Monophosphate Dehydrogenase
Xuan, Xuenan; Nishikawa, Yoshifumi; Igarashi, Ikuo; Masatani, Tatsunori; Moumouni, Paul Franck Adjou; Kamyingkird, Ketsarin; Zhou, Mo; Terkawi, Mohamad Alaa; Aboge, Gabriel Oluga; Cao, Shinuo (2014)The resistance of Babesia parasites to current anti-babesiosis drugs is an issue of major concern. The inosine 5'-monophosphate dehydrogenase (IMPDH) of Babesia gibsoni has been identified and characterized as a molecular ... -
A novel 57-kDa merozoite protein of Babesia gibsoni is a prospective antigen for diagnosis and serosurvey of canine babesiosis by enzyme-linked immunosorbent assay
Aboge, Gabriel Oluga; Jia, Honglin; Goo, Younkyoung; Kuriki, Ken; Nishikawa, Yoshifumi; Igarashi, Ikuo; Suzuki, Hiroshi; Xuan, Xuenan (Department of Public Health Pharmacology and Toxicology, 2007)We isolated a novel single copy gene encoding a 57-kDa merozoite protein of Babesia gibsoni (BgP57). The nucleotide sequence of the cDNA was 2387 bp with an open reading frame (ORP) of 1644 bp encoding a 57-kDa predicted ... -
Prime-boost vaccination with plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs
Terkawi, Mohamad A; Moumouni, Paul F A; Zhou, Mo; Kamyingkird, Ketsarin; Aboge, Gabriel O; Mousa, Ahmed A; Shinuo, Cao; Xuan, Xuenan; Fukumoto, Shinya; Suzuki, Hiroshi; Masatani, Tatsunori; Yoshifumi, Nishikawa (2013)A heterologous prime-boost vaccination regime with DNA and recombinant vaccinia virus (rvv) vectors expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In ... -
Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice
Yu, Longzheng; Yamagishi, Junya; Zhang, Shoufa; Jin, Chunmei; Aboge, Gabriel Oluga; Zhang, Houshuang; Zhang, Guohong; Tanaka, Tetsuya; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan (2012)A heterologous prime-boost strategy with priming plasmid DNA followed by recombinant virus expressing relevant antigens is known to stimulate protective immunity against intracellular parasites. In this study, we have ... -
Toxoplasma gondii: Sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method
Zhang, Houshuang; Thekisoe, Oriel M.M; Aboge, Gabriel O; Kyan, Hisako; Yamagishi, Junya; Inoue, Noboru; Nishikawa, Yoshifumi; Zakimi, Satoshi; Xuan, Xuenan (2009)Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high specificity, sensitivity and rapidity. In this study, we used a conserved sequence in the 200- to 300-fold repetitive 529 bp gene of Toxoplasma ...