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Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand
(2012)
Although Babesia bovis and Babesia bigemina infections cause economic losses in the cattle industry in northern Thailand, there is inadequate information on Babesia isolates present in the area. Therefore, to determine the ...
Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen
(2008)
A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library
with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of
762 bp and 254 ...
Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice
(2012)
A heterologous prime-boost strategy with priming plasmid DNA followed by recombinant virus expressing relevant antigens is known to stimulate protective immunity against intracellular parasites. In this study, we have ...
Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni
(2012)
We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, ...
Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family
(2012)
We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding ...
Mycophenolic Acid, Mycophenolate Mofetil, Mizoribine, Ribavirin, and 7-Nitroindole Inhibit Propagation of Babesia Parasites by Targeting Inosine 5'-Monophosphate Dehydrogenase
(2014)
The resistance of Babesia parasites to current anti-babesiosis drugs is an issue of major concern. The inosine 5'-monophosphate dehydrogenase (IMPDH) of Babesia gibsoni has been identified and characterized as a molecular ...
Macrophages Are Critical for Cross-Protective Immunity Conferred by Babesia microti against Babesia rodhaini Infection in Mice
(2012)
AlthoughprimaryinfectionofmicewithBabesiamicrotihasbeenshowntoprotectmiceagainstsubsequentlethalinfection by Babesiarodhaini,themechanismbehindthecross-protection is unknown.To unravel lthis mechanism,we investigated the ...
Toxoplasma gondii: Sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method
(2009)
Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high specificity, sensitivity and rapidity. In this study, we used a conserved sequence in the 200- to 300-fold repetitive 529 bp gene of Toxoplasma ...