Epitope cross-reactivity frequently differs between central and effector memory HIV-specific CD8+ t cells1
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Date
2007-03Author
McKinnon, Lyle R
Ball, T Blake
Wachihi, Charles
McLaren, Paul J
Waruk, Jillian LM
Mao, Xiaojuan
Ramdahin, Sue
Anzala, A Omu
Kamene, Jane
Luo, Ma
Fowke, Keith R
Plummer, Francis A
Language
enMetadata
Show full item recordAbstract
HIV diversity may limit the breadth of vaccine coverage due to epitope sequence differences between strains. Although amino acid substitutions within CD8+ T cell HIV epitopes can result in complete or partial abrogation of responses, this has primarily been demonstrated in effector CD8+ T cells. In an HIV-infected Kenyan cohort, we demonstrate that the cross-reactivity of HIV epitope variants differs dramatically between overnight IFN-γ and longer-term proliferation assays. For most epitopes, particular variants (not the index peptide) were preferred in proliferation in the absence of corresponding overnight IFN-γ responses and in the absence of the variant in the HIV quasispecies. Most proliferating CD8+ T cells were polyfunctional via cytokine analyses. A trend to positive correlation was observed between proliferation (but not IFN-γ) and CD4 counts. We present findings relevant to the assessment of HIV vaccine candidates and toward a better understanding of how viral diversity is tolerated by central and effector memory CD8+ T cells.
Citation
The Journal of Immunology March 15, 2007 vol. 178 no. 6 3750-3756Publisher
Univerisyt of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10378]