Epitope Cross-Reactivity Frequently Differs between Central and Effector Memory HIV-Specific CD8 T Cells1
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Date
2007Author
McKinnon, Lyle R.
Ball, T. Blake
Wachihi, Charles
McLaren, Paul J.
Waruk, Jillian L. M.
Mao, Xiaojuan
Ramdahin, Sue
Anzala, A. Omu
Kamene, Jane
Luo, Ma
Fowke, Keith R.
Plummer, Francis A.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
HIV diversity may limit the breadth of vaccine coverage due to epitope sequence differences between strains. Although amino acid
substitutions within CD8 T cell HIV epitopes can result in complete or partial abrogation of responses, this has primarily been
demonstrated in effector CD8 T cells. In an HIV-infected Kenyan cohort, we demonstrate that the cross-reactivity of HIV epitope
variants differs dramatically between overnight IFN- and longer-term proliferation assays. For most epitopes, particular variants
(not the index peptide) were preferred in proliferation in the absence of corresponding overnight IFN- responses and in the
absence of the variant in the HIV quasispecies. Most proliferating CD8 T cells were polyfunctional via cytokine analyses. A trend
to positive correlation was observed between proliferation (but not IFN- ) and CD4 counts. We present findings relevant to the
assessment of HIV vaccine candidates and toward a better understanding of how viral diversity is tolerated by central and effector
memory CD8 T cells.
Citation
The Journal of Immunology, 2007, 178: 3750–3756.Collections
- Faculty of Health Sciences (FHS) [10377]