Search
Now showing items 1-10 of 12
Epitope Mapping of HIV-Specific CD8+ T cells in a Cohort Dominated by Clade A1 Infection
(2009)
Background
CD8+ T cell responses are often detected at large magnitudes in HIV-infected subjects, and eliciting these responses is the central aim of many HIV-1 vaccine strategies. Population differences in CD8+ T cell ...
Genetic analysis of human DNA recovered from minute amounts of serum or plasma
(University of Nairobi, 1995-03-13)
A rapid and inexpensive method is described where a small amount of serum or plasma was used as the source of DNA for genetic analysis. Using a silica gel matrix DNA was isolated from 50 μl of archived serum or plasma. The ...
The role of G protein gene GNB3 C825T Polymorphism in HIV-1 acquisition, progression and immune activation
(2012)
Background
The GNB3 C825T polymorphism is associated with increased G protein-mediated signal transduction, SDF-1α-mediated lymphocyte chemotaxis, accelerated HIV-1 progression, and altered responses to antiretroviral ...
Epitope cross-reactivity frequently differs between central and effector memory HIV-specific CD8+ t cells1
(Univerisyt of Nairobi, 2007-03)
HIV diversity may limit the breadth of vaccine coverage due to epitope sequence differences between strains. Although amino acid substitutions within CD8+ T cell HIV epitopes can result in complete or partial abrogation ...
Reduced Cellular Susceptibility to In Vitro HIV Infection Is Associated with CD4+ T Cell Quiescence
(2012)
Background
HIV preferentially establishes productive infection in activated CD4+ T cells. Since proportions of activated CD4+ T cells vary between individuals, this study aimed to determine if individuals with a greater ...
Elevated expression of LAG-3, but not PD-1, is associated with impaired iNKT cytokine production during chronic HIV-1 infection and treatment
(University of Nairobi, 2015)
Background:
LAG-3 is a potent negative regulator of the immune response but its impact in HIV infection in
poorly understood. Unlike exhaustion markers such as PD-1, Tim-3, 2B4 and CD160, LAG-3 is poorly expressed on
bulk ...
Enrichment of LAG-3, but not PD-1, on Double Negative T Cells at the Female Genital Tract
(University of Nairobi, 2014-12)
Problem
The expression of inhibitory markers such as LAG-3 and PD-1 on T lymphocytes regulates immune function. Their expression at the genital mucosa is poorly understood, but regulation of immune activation at the ...
The impact of sex work interruption on blood-derived t cells in sex workers from Nairobi, Kenya
(University of Nairobi, 2016)
Background: Unprotected sexual intercourse exposes the female genital tract (FGT) to semen-derived antigens, which leads to a proinflammatory response. Studies have shown that this postcoital inflammatory response can lead ...
Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract
(2010)
Plant extracts with antioxidant activity could also have immunomodulatory ability. The free radical scavenging activity of an ethanol extract of the leaves of Stachytarpheta angustifolia was assessed by measuring its ...
HIV Type 1 Resistance in Kenyan Sex Workers Is Not Associated with Altered Cellular Susceptibility to HIV Type 1 Infection or Enhanced β-Chemokine Production
(1998)
A small group of women (n = 80) within the Nairobi-based Pumwani Sex Workers Cohort demonstrates epidemiologic resistance to HIV-1 infection. Chemokine receptor polymorphisms and β-chemokine overproduction have been among ...